Vlastislav Matoušek studied composition and music theory at the AMU Faculty of Music, where in 2001 he defended his dissertation on Kinetics in ethnic music and received a doctorate in the field of music theory, in 2004 he was appointed associate professor. He lectured on music-theoretical subjects, especially ethnomusicology, at the Department of Music Theory at HAMU and the Institute of Musicology of the Faculty of Arts, Charles University in Prague. In 1996, as a Japan Foundation scholarship holder, he studied shakuhachi flute with Kifu Mitsuhashi in Tokyo. He worked as a music columnist with Czech Radio. He is the author of many compositions, often with the participation of exotic instruments and electronics. He performs concerts on the shakuhachi, presents contemporary music and his own compositions with his Ensemble 108 Hz. He plays and sings music from the Gothic period with the family ensemble Schola Specialis Familiae on historical instruments.